النتائج (
الإنجليزية) 3:
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2.2. Antimicrobial effect assaysA suitable aliquot of fresh spore suspensions of P.expansum was added to a 250 mL conical flask contain-ing 100 mL potato dextrose broth (PDB) to obtain a finalconcentration of 1 x 106spores / mL with the aid of a hemo-cytometer. NaHCO3was, respectively, added to the medium withfinal concentrations 0, 0.15%, 0.3%, 0.45%, 0.6% (M / V). After shakingculture at 25 ◦ C, at least 100 spores per replicate were observed microscopically to determine germination rate during 6 to 10 hand germ tube length during 11 to 14 h.
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