النتائج (
الإنجليزية) 2:
[نسخ]نسخ!
Genome editing allows precise DNA manipulation, but its potential is limited in many crops by<br>22 low regeneration efficiencies and few transformable genotypes. Here, we show that expression<br>23 of a chimeric protein including wheat GROWTH-REGULATING FACTOR 4 (GRF4) and its<br>24 cofactor GRF-INTERACTING FACTOR 1 (GIF1) dramatically increases the efficiency and<br>25 speed of regeneration in wheat, triticale and rice and expands the number of transformable<br>26 wheat genotypes. Moreover, GRF4-GIF1 induces efficient wheat regeneration in the absence of<br>27 exogenous cytokinins, which facilitates selection of transgenic plants without selectable<br>28 markers. By combining GRF4-GIF1 and CRISPR-Cas9 technologies, we were able to generate<br>29 large numbers of edited wheat plants. The GRF4-GIF1 transgenic plants were fertile and<br>30 without obvious developmental defects, likely due to post-transcriptional regulatory<br>31 mechanisms operating on GRF4 in adult tissues. Finally, we show that a dicot GRF-GIF<br>32 chimera improves regeneration efficiency in citrus suggesting that this strategy can be<br>33 expanded to dicot crops.
يجري ترجمتها، يرجى الانتظار ..
![](//arimg.ilovetranslation.com/pic/loading_3.gif?v=b9814dd30c1d7c59_8619)