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Evidence from which this concept evolved. SteinAnd Stein localized radioautographic grains overSubcellular structures after the intravascular injectionOf SH] [glycerol and palmitic acid. AlthoughThe interpretation was made initially that grainsWere localized over the rough endoplasmic reticulumBy 2 and 5 min, the published images showGrains only in the vicinity of the ends of parallel arraysOf RER or of single RER cisternae, and noGrains were clearly associated with flattened cisternaeContaining ribosomes (49). Although theGrain size was too large for precise localizationThese investigators have recently stated thatGrains were seen often in the regions of transitionBetween the rough and smooth ER (50). ThusContrary to previous interpretations, the radioautographicStudies suggest to us that the ribosome.Studded, flattened cisternae of the roughEndoplasmic reticulum do not synthesize triglycerides.The presence of label clearly localized inRegions of transition between smooth and roughEndoplasmic reticulum, particularly at the earliestTimes of sampling of liver (2-5 min after injection),Suggests that triglyceride synthesis occursEither in this transitional compartment or inNearby smooth ER cisternae (and is then transported
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